CELL AND NUCLEIC ACID THERAPEUTICS COOP - Spring House, PA
Location:
Spring House , Pennsylvania
Posted:
February 28, 2017
Reference:
8348170130-en-us

The candidate will contribute to research efforts related to the:

  1. Production and purification of AAV, and the
  2. The optimization of nucleic-acid delivery platforms such as Adeno-Associated Virus (AAV) and nanoparticle technologies.

The scientist will subsequently characterize gene delivery results and the functionality of cellular products via an array of biological assays.

 
The candidate will learn about and perform tasks including:
  • Maintenance of cell lines,
  • Transfection of plasmid DNA to generate virus,
  • Purification techniques including affinity/IEX chromatography,
  • Assays to validate the success of purification, assays to assess infectivity and gene delivery of virus. 
Techniques the candidate would learn include:
  • Digital drop PCR,
  • Silver staining/Western blotting,
  • FPLC-based methods: affinity and Ion-exchange chromatography, cell culture, confocal imaging, and much more.

Qualifications
  • Candidates must be currently enrolled in an accredited college/institution pursuing a Bachelor’s or Master’s degree in Pre-Medicine, Biology, Biochemistry or any Life Science related field.
  • Preferred minimum 3.0 GPA
  • An understanding of basic molecular biology and immunology is required.
  • Experience in mammalian cell culture and routine biological assays and data interpretation is preferred. 
  • The successful applicant is required to independently study and understand the basic underlying science behind all tasks performed and ask questions necessary to understand the work.
  • The successful candidate will initially work cooperatively with a mentor but is expected to be able to independently carry out most tasks after a training period.
  • The ability to generate and maintain experimental data in an electronic laboratory notebook in a highly organized manner is essential. 

Primary Location
United States-Pennsylvania-Spring House
Organization
Janssen Research & Development, LLC. (6084)
Job Function
Administration

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