The candidate will contribute to research efforts related to
and purification of AAV, and the
optimization of nucleic-acid delivery platforms such as Adeno-Associated Virus
(AAV) and nanoparticle technologies.
The scientist will subsequently characterize gene delivery
results and the functionality of cellular products via an array of biological
The candidate will learn about and perform tasks including:
- Maintenance of cell lines,
- Transfection of plasmid DNA to generate virus,
- Purification techniques including affinity/IEX
- Assays to validate the success of purification,
assays to assess infectivity and gene delivery of virus.
Techniques the candidate would learn include:
- Digital drop PCR,
- Silver staining/Western blotting,
- FPLC-based methods: affinity and Ion-exchange
chromatography, cell culture, confocal imaging, and much more.
- Candidates must be currently enrolled in an
accredited college/institution pursuing a Bachelor’s or Master’s degree in
Pre-Medicine, Biology, Biochemistry or any Life Science related field.
- Preferred minimum 3.0 GPA
- An understanding of basic molecular biology and
immunology is required.
- Experience in mammalian cell culture and routine
biological assays and data interpretation is preferred.
- The successful applicant is required to
independently study and understand the basic underlying science behind all
tasks performed and ask questions necessary to understand the work.
- The successful candidate will initially work
cooperatively with a mentor but is expected to be able to independently carry
out most tasks after a training period.
- The ability to generate and maintain
experimental data in an electronic laboratory notebook in a highly organized
manner is essential.
United States-Pennsylvania-Spring HouseOrganization
Janssen Research & Development, LLC. (6084)Job Function