A career at Pfizer offers opportunity, ownership and impact. All over the world, Pfizer colleagues work together to positively impact health for everyone, everywhere. Our colleagues have the opportunity to grow and develop a career that offers both individual and company success; be part of an ownership culture that values diversity and where all colleagues are energized and engaged; and the ability to impact the health and lives of millions of people. Pfizer, a global leader in the biopharmaceutical industry, is continuously seeking top talent who are inspired by our purpose to innovate to bring therapies to patients that significantly improve their lives. Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer. Colleagues who are issued an Incident Final Warning (IFW) on or after January 1, 2016, are not eligible to post and compete for a position for a period of 12-months from the date an IFW is issued. We are seeking a highly motivated scientist to join the Target Discovery Group in Pfizer's Oncology Research &; Development organization. The successful applicant will employ multiple genomic and functional genomic platforms to identify and validate novel small molecule or antibody drug targets. Biological focus will be in the area of identifying synthetic lethal oncology targets arising from tumor genetic vulnerabilities, and novel targets in immuno-oncology. Responsibilities The successful candidate will work independently as part of a research team that is focused on identifying and validating novel cancer drug targets. The candidate is expected to utilize expertise in molecular and cellular biology, cancer biology, and functional genomics to execute and interpret experiments to develop targets for therapeutic intervention. Specific technical duties include: Utilize molecular and cellular biology skills including DNA cloning, cell culture, lentiviral shRNA and CRISPR knockdown technologies followed by global MiSeq analysis to execute large-scale genomic screens. Apply both genetic and pharmacologic approaches to modulate target candidates in vitro and assessing the functional impact on cancer cell biology. These include RNAi, CRISPR, small molecules, antibodies and other pharmacologic approaches to modulate targets in vitro followed by molecular and phenotypic analysis. Execute molecular and cell-based assays across a wide variety of technical platforms (qPCR, Western blotting, FACS, immunofluorescence, ELISA, HCS etc.) to assess target modulation and its impact on cell viability and cell cycle disruption, downstream signal transduction, transcription and immune susceptibility. Utilize expertise in cancer biology to design, execute, and analyze experiments to advance mechanism of action target biology and develop innovative approaches for targeting cancer. Utilize electronic data capture/analysis tools such as Excel, GraphPad, Biobook, Spotfire and PowerPoint for experimental documentation and data analysis/presentations Present experimental results at both internal group meetings and meetings with members of the Oncology Research &; Development organization. Educational Requirements: BS in Molecular Biology, Cell Biology, Cancer Biology (or a comparable biological science) with 6+ years of experience in academia or industry focused on cancer biology and drug discovery, or MS with 5+ years. Extensive experience with molecular and cell culture techniques for gene modulation and downstream molecular analysis Technical Skill Requirements: Technical knowledge of cancer biology and oncogenic signaling pathways is required. Expertise in mammalian cell culture techniques and molecular biology required. Expertise in gene knockdown technologies utilizing RNAi and CRISPR platforms, gene expression analysis (qPCR, Western blotting, FACS analysis) and cell-based phenotypic assays is critical. Strong track record in screening approaches for target identification, utilization of inhibitors or genetic manipulations for target validation and/or expertise in immune-oncology. Excellent verbal/written communication and interpersonal skills with an emphasis on working in a team environment to accomplish impactful results. Ability to work in a fast paced dynamic environment. PHYSICAL/MENTAL REQUIREMENTS Job requires laboratory work which includes extended periods of sitting and standing A career at Pfizer offers opportunity, ownership and impact. All over the world, Pfizer colleagues work together to positively impact health for everyone, everywhere. Our colleagues have the opportunity to grow and develop a career that offers both individual and company success; be part of an ownership culture that values diversity and where all colleagues are energized and engaged; and the ability to impact the health and lives of millions of people. Pfizer, a global leader in the bio-pharmaceutical industry, is continuously seeking top talent who are inspired by our purpose to innovate to bring therapies to patients that significantly improve their lives. Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.